As the leading manufacturer of zebrafish antibodies, genetex offers an extensive portfolio of reagents validated for use in this model system. Immunohistochemistry of wholemount and sectioned zebrafish tissue. The nsmase2 protein is a reported synonym for the human gene smpd3, encoding sphingomyelin phosphodiesterase 3. Due to their striking genomic similarity to humans, regenerative abilities, and shortened life cycle, zebrafish have become an ideal tool for studying developmental processes, gene expression, and. Overexpression of serbp1 plasminogen activator inhibitor 1. Zebrafish antibodies primary antibodies sigmaaldrich. A guide for the laboratory use of zebrafish danio rerio, eugene, university of oregon press. Regulation of 53bp1 protein stability by rnf8 and rnf168 is important for efficient dna doublestrand break repair article pdf available in plos one 910. The tumor suppressor protein p53, a sequence specific transcription factor, plays a central role in the. Select, antibody name, antigen gene name, isotype balbc mice, cellular binding site, recommended dilution, anatomical structure labeled, availability.
Casp1, caspase 1, apoptosisrelated cysteine peptidase interleukin 1, beta, caspase 1, apoptosisrelated cysteine protease interleukin 1, beta, caspase1, ec 3. Zebrafish danio rerio, a minnowlike freshwater fish, has emerged as a popular animal model for research and development. Regulation of 53bp1 protein stability by rnf8 and rnf168. Zirc monoclonal antibodies zebrafish international resource. B the 3d larvae labeled with antiacetylated tubulin antibody brown, lateral view with anterior to the left. Mos have been used to accelerate gene discovery through largescale screening, 1,2 to probe candidate gene function, 3 and to verify mutant phenotypes.
Zebrafish p53 is highly similar to mammalian p53 in both structure and function. Retinal precursor cells mutant for caf1b arrest in s phase and undergo p53mediated apoptosis. In a recent study, percival and co have developed a novel technique utilizing the natural transparency of zebrafish embryos, allowing to observe chromosome dynamics within a single cell during mitosis in a. Our team decided to produce antibodies against key immune molecules in zebrafish to enhance the research within the fish. Serbp1, a protein that is supposed to regulate the. The zebrafish book a guide for the laboratory use of.
Due to their striking genomic similarity to humans, regenerative abilities. The nsmase2 protein is a reported synonym for the human gene smpd3, encoding. Antinsmase2 antibody products antinsmase2 antibodies can be readily obtained from commercial sources. It relocates to the sites of dna strand breaks in response to dna damage and is a putative substrate of the ataxia telangiectasiamutated atm kinase. Exploring zebrafish genomic, functional and phenotypic data using. Zebrafish are an ideal model system for studying the adaptive immune system because in evolutionary terms they have the earliest recognizable adaptive immune system whose features match the essential human elements 7, 8. The juxtamembrane mutation of flt3 is the strongest independent marker for disease relapse and is associated with elevated bcl2 protein and p53 hyperphosphorylation in aml. Maternal transfer and protective role of antibodies in zebrafish danio rerio. Dna damage forms the basic mechanism of cancer cell eradication in. Nb10056565 caspase1 antibody 14f468 novus biologicals. Pdf regulation of 53bp1 protein stability by rnf8 and. Analysis of p53 status in human cell lines using a functional. Regulation of 53bp1 protein stability by rnf8 and rnf168 is.
About these antibodies all antibodies listed on this page were generated by researchers at the institute of neuroscience, university of oregon liu et al. Live imaging of endogenous protein dynamics in zebrafish using chromobodies. Overexpression of serbp1 plasminogen activator inhibitor. Blots were then incubated for 1 h at room temperature with dako peroxidaseconjugated rabbit antimouse immuno globulin diluted 1. Plasminogen activator inhibitor 1 pai1 overexpression is an important prognostic and predictive biomarker in human breast cancer. The 53bp1 protein is a reported synonym for the human gene tp53bp1, encoding tumor protein p53 binding protein 1. The monoclonal antibodies were generated with balbc mice against adult zebrafish protein, and clones were selected based on the specific staining patterns obtained with particular. May 11, 2007 acute myeloid leukemia aml cells are characterized by nonmutated tp53, high levels of hdm2, and frequent mutation of the flt3 receptor tyrosine kinase.
As such, zfin provides a centralized resource for zebrafish genomic, genetic. Sectioning of embryos or larvae staining sections with antibodies using pap. The tumor suppressor protein p53, a sequence specific transcription factor, plays a central role in the response of mammalian cells to genotoxic stress. Antibody nb600242 foxp3 antibody by novus biologicals.
The importance of p53 in cancer is highlighted by the fact that it is the most frequently mutated tumor suppressor gene in human cancers, with more. A 28h wholemount zebrafish embryo labeled with an antibody which recognizes the transcription factor, pax6 brown label showing a lateral view with anterior to the left. Anti53bp1 antibodies can be readily obtained from commercial sources. The fulllength protein is 2,574 daltons in mass with 3 identified isoforms. Zebrafish antibodies zebrafish danio rerio, a minnowlike freshwater fish, has emerged as a popular animal model for research and development. Maternal transfer and protective role of antibodies in zebrafish danio.
Highthroughput sequencing of the zebrafish antibody. Analysis of p53 status in human cell lines using a. Serbp1, a protein that is supposed to regulate the stability of pai1 mrna, may play a role in gynaecological cancers as well, since upregulation of serbp1 was described in ovarian cancer recently. Like humans, zebrafish have a recombination activating gene rag and a combinatorial rearrangement of v, d and j gene segments to create antibodies. The zebrafish book a guide for the laboratory use of zebrafish danio brachydanio rerio. The zebrafish book a guide for the laboratory use of zebrafish danio brachydanio rerio by monte westerfield, institute of neuroscience, university of oregon. Fulllength zebrafish p53 is 48% identical in amino acid sequence to human p53 cheng et al. M orpholino oligonucleotides mos are the most widely used antisense knockdown tools in the zebrafish danio rerio community. It relocates to the sites of dna strand breaks in response to dna damage and is a putative substrate. Dec, 2012 plasminogen activator inhibitor 1 pai1 overexpression is an important prognostic and predictive biomarker in human breast cancer. Blots were then incubated for 1 h at room temperature with dako peroxidase. Oct, 2019 results demonstrate that zebrafish p53 protein can readily be induced in embryos and detected using these new antibody tools, which will increase the usefulness of zebrafish as a model in compoundbased screening for novel drugs in cancer research.
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